Senior Associate Scientist, Biochemistry

Job description

Employer Name: Alphazyme LLC Title: Senior Associate Scientist, Biochemistry: Duties: Support microbial strain and product development pipelines through strain engineering and gene expression strategies. Support storage and maintenance of microbial stock cultures, ensuring proper documentation and viability. Design and perform basic molecular biology workflows, including PCR, Western blot, ELISA, gel electrophoresis, nucleic acid purification, gene assembly and cloning. Develop and execute experiments for enzyme engineering and optimization, including directed evolution and rational design approaches. Develop and validate high-throughput screening assays for enzyme activity, stability, and specificity. Collaborate with cross-functional teams, including process development and manufacturing, to support scale-up and technology transfer. Express protein production in bacterial and fungal hosts to improve yield and functionality. Perform and interpret basic biochemical analyses, including protein gel electrophoresis, Western blotting, fluorescence labeling, and activity assays. Develop and implement structured training programs for new Research and Development (R&D) staff members. Assi...